Polymerase Chain Reaction

PolymeraseChain Reaction

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Whatis PCR? Describe the technique in detail

Alsoknown as was established in the 1980s. Thetechnique allows utilization of the DNA polymerase ability ofsynthesizing new DNA strand, which complements the template strand(DNA Learning Center, 2014). The ability of this technique is thenature of DNA polymerase whereby a nucleotide can only be added onto3’-OH group and any other additional nucleotide requires a primer(University of Utah, 2014). The requirement of primer allows aresearcher to delineate a specified region of template sequence toamplify. Through the PCR reaction, numerous copies (amplicons) areaccumulated.

Whatenzyme is used in this process and how is the enzyme used in PCRdifferent from others?

Theenzyme that is important is the DNA polymerase (NCBI, 2014). Theadvantages of these enzymes when compared to others include its heatresistant nature and utilization of primers and DNA template togenerate a new strand (University of Utah, 2014).

Whatis that enzyme`s function in the cell?

Twotypes of enzymes exist, which are Taq DNA and Pfu DNA. The Taq DNA iscommonly utilized while Pfu DNA is employed because of higherfidelity during DNA copying (University of Utah, 2014). Both types ofenzymes allow synthesis of new strands of DNA complementary to thespecified sequence (DNA Learning Center, 2014).

Howhas PCR changed microbiology?

Someof the benefits associated to PCR technique is its ability to makenumerous copies starting with minimal amount of DNA (DNA LearningCenter, 2014). In addition, the process is speedy and hence manycopies can be created within a short time when compared to othertechniques (University of Utah, 2014). Therefore, PCR has changed andimproved microbiology because the researchers can utilize andgenerate many samples based on a small template.

References

DNALearning Center. (2014). Polymerasechain reaction.Retrieved from http://www.dnalc.org/resources/animations/pcr.html

NCBI.(2014). PCR.Retrieved fromhttp://www.ncbi.nlm.nih.gov/projects/genome/probe/doc/TechPCR.shtml

Universityof Utah. (2014). PCR.Retrieved from http://learn.genetics.utah.edu/content/labs/pcr/